93363
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93363
Sigma
Trizma® hydrochloride
BioUltra, for molecular biology, ≥99.0% (AT)
| Synonym: | TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride |
| CAS Number: | 1185-53-1 |
| Linear Formula: | NH2C(CH2OH)3 · HCl |
| Molecular Weight: | 157.60 |
| Beilstein Registry Number: | 3675235 |
| EC Number: | 214-684-5 |
| MDL number: | MFCD00012590 |
| PubChem Substance ID: | 24889855 |
Description
| Application | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Other Notes | Component of extraction buffer e.g. phenol extraction of DNA or RNA1; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE2. |
| Easily compare specifications for Trizma HCl products with the Trizma HCl specification table. | |
| Legal Information | Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | for molecular biology |
| product line | BioUltra |
| assay | ≥99.0% (AT) |
| total impurities | DNases, none detected |
| RNases, none detected | |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| ign. residue | ≤0.2% (as SO4) |
| loss | ≤0.2% loss on drying, 110 °C |
| pH | 2.5-4.0 (25 °C, 4 M in H2O) |
| useful pH range | 7.0 - 9.0 |
| pKa (25 °C) | 8.1 |
| solubility | H2O: 4 M at 20 °C, clear, colorless |
| anion traces | sulfate (SO42-): ≤50 mg/kg |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| λ | 4 M in H2O |
| UV absorption | λ: 260 nm Amax: 0.12 |
| λ: 280 nm Amax: 0.08 |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 1 |
